Overview
Processing many times uses high value enzymes which act like catalysts. The effective recycling helps companies increase their profitability and operating costs.This recycle can be acheived by our Poresep range of membranes. Poresep Membrane filtration is used to concentrate and purify enzymes in manufacturing and also recycle enzyme and water in the production process.

Applications
Protease
Amylase
Cellulase
Lipase
Lactase
Rennet
Trypsin
Pepsin
Bromelain
Papain
Pectin
Lysozyme

Feature and Advantages

  • Multiple operations like Concentration and purification in single continuous step
  • Non-Chemical reduction of salts, colour and pyrogens using diafiltration
  • High yield, even at aslow temperatures as 4-5 Deg C
  • Efficient energy utilisation
  • Purity acheivable below ppm levels
  • Higher enzyme concentration when combining ultrafiltration and nanofiltration processes
  • Operation possible at low temperature eliminating product deterioration for thermally unstable products
  • Sanitary designs
  • Bespoke designs

Process Operation

Enzymes are manufactured using processes like fermentation, extraction for plant and animal tissues as intracellular products or as extracellular. The challenge in processing of enzymes is that the solutions have unwanted contaminants which are low molecular weight compounds such as salts or metabolic products.

Poresep Ultrafiltration is used as a technique to remove the above contaminants or impurities. Membrane processing is a gentle and a low temperature process to do molecular separation, purification and concentration in a single step. Concentrations as high as 20x – 25x can be achieved without much loss of activity.  The enzymes are purified by simple UF and dia-filtration techniques which increases the purity still further, reducing the salts, colours and even endotoxins.

Prosep membrane systems  operate using  membrane elements of different MOC  that are configured  in typical crossflow mode. That technology purely relies on the differences in physical sizes in the MF range and in the molecular weight (size) of the individual components when processing in the MF/NF and RO range.  The membrane pore is a safe barrier for any insoluble matter (i.e. protein complexes if any,  triglycerides, fats, oils, bacteria, colloidal matter, fibres etc) irrespective of the incoming concentrations. The selection of the right membrane is more from experience of working in similar applications.

Technical Specification
Membrane types available suitable for a wide range of enzyme applications and process conditions.

NF MEMBRANES :  NF Membranes- Available in different cut-offs
Modified Polyacrylonitrile, Ceramic, PES – MW- 100 dalton,  200 dalton, 300 dalton, 400 dalton
UF/MF  MEMBRANES: Polyamide, PES,  Cellulose,  Low Protien Binding Polysulfone, Ceramic, Stainless Steel
MW Cut-Off or Micron : 1,000 MW, 5,000 MW,  10,000 MW,  20,000 MW, 25,000 MW, 30,000 MW, 50,000 MW,  100,000 MW,  120,000 MW